The answer to the crossword clue "It dots the "i" in the Ohio State marching band's spelling of "Ohio" is sousaphone. The sousaphone is a large, tuba-like instrument that is played by the bass section of the marching band. The player of the sousaphone stands in the middle of the "O" in the word "Ohio" and holds the instrument up so that it dots the "i".
Here are some other possible answers to the clue, depending on the number of letters required:
5 letters: TUBA
6 letters: INSTRUMENT
7 letters: BASS SECTION
8 letters: MARCHING BAND
The best answer will depend on the specific crossword puzzle and the other clues that are present.
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